cd4 cd25 foxp3 treg isolation kit  (Miltenyi Biotec)

Journal: Nature Communications

Article Title: Increased SOAT2 expression in aged regulatory T cells is associated with altered cholesterol metabolism and reduced anti-tumor immunity

doi: 10.1038/s41467-025-56002-w

Figure Lengend Snippet: A Diagram of murine CD4 + CD25 + , CD4 + CD25 - T or CD4 - lymphocytes isolated by magnetic bead sorting from LSCC tumor tissue and spleen samples (Created in BioRender. Major, Z. (2024) https://BioRender.com/u18t594 ). B , C The proteins extracted from tumor ( B ) and spleen ( C ) Lymphocyte subsets in indicated groups were assessed by Western blotting analysis. The experiment was repeated 3 times independently with similar results. D , E The SOAT2 expression was assessed in tumor and spleen CD4 + CD25 + T lymphocytes by flow cytometry assay ( D ); quantitation of SOAT2 expression was shown ( n = 5) ( E ). F , G The SOAT2 expression was assessed in tumor and spleen CD4 + CD25 - &CD4 - T lymphocytes by flow cytometry assay ( F ); quantitation of SOAT2 expression was shown ( n = 5) ( G ). H Diagram of human CD4 + CD25 + T lymphocytes, CD4 + CD25 - T or CD4 - lymphocytes isolated by magnetic bead sorting from blood samples (Created in BioRender. Major, Z. (2024) https://BioRender.com/w00f660 ). I , J The mRNA and protein expression of SOAT2 in indicated groups were detected by quantitative RT-PCR ( I ) and Western blotting ( J , The experiment was repeated 3 times independently with similar results) analysis. Data represented means ± SD. Statistical difference was evaluated by unpaired two-tailed student’s t -test ( E , G ), and one-way ANOVA test ( I ). Source data are provided as a Source Data file. (* P < 0.05; *** P < 0.001; ns no significance).

Article Snippet: For mouse samples, CD4 + CD25 + FOXP3 + T lymphocyte cells (Treg cells), CD4 − T lymphocyte cells, CD8 + TILs (Teffs) were enriched and sorted from spleen and tumor tissues using a modified isolation protocol by Regulatory T Cell Isolation Kit and CD8 + TILs Isolation Kit (#130-091-041, #130-116-478, Miltenyi Biotec).

Techniques: Isolation, Western Blot, Expressing, Flow Cytometry, Quantitation Assay, Quantitative RT-PCR, Two Tailed Test

Journal: Nature Communications

Article Title: Increased SOAT2 expression in aged regulatory T cells is associated with altered cholesterol metabolism and reduced anti-tumor immunity

doi: 10.1038/s41467-025-56002-w

Figure Lengend Snippet: A – D Ki-67 staining in indicated groups was analyzed by flow cytometry to measure lymphocyte proliferation ( A , C ); quantitation of Ki67 of Max (%) was shown ( n = 5) ( B , D ). E , F The apoptotic Treg cells in indicated groups were analyzed by flow cytometry with 7-AAD and PE-Annexin V staining; the percentage of apoptotic Treg cells in indicated groups were shown ( n = 3). G , H The migration of Treg cells in indicated groups were detected by transwell assays ( n = 5). I , J FOXP3 staining in indicated groups was analyzed by flow cytometry to measure Treg cells stability. K The differentiation ability of naive CD4 + T cells to CD4 + CD25 + FOXP3 + T lymphocytes sorted in indicated groups under Treg polarizing conditions was detected by flow cytometry assay. Data represented means ± SD. Statistical difference was evaluated by one-way ANOVA test ( B , D , E , F , G , H ). Source data are provided as a Source Data file. (* P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: For mouse samples, CD4 + CD25 + FOXP3 + T lymphocyte cells (Treg cells), CD4 − T lymphocyte cells, CD8 + TILs (Teffs) were enriched and sorted from spleen and tumor tissues using a modified isolation protocol by Regulatory T Cell Isolation Kit and CD8 + TILs Isolation Kit (#130-091-041, #130-116-478, Miltenyi Biotec).

Techniques: Staining, Flow Cytometry, Quantitation Assay, Migration